diff --git a/src/ImageSharp/Memory/Allocators/UniformUnmanagedMemoryPoolMemoryAllocator.cs b/src/ImageSharp/Memory/Allocators/UniformUnmanagedMemoryPoolMemoryAllocator.cs
index 16a3cb73d..0da4ff9f8 100644
--- a/src/ImageSharp/Memory/Allocators/UniformUnmanagedMemoryPoolMemoryAllocator.cs
+++ b/src/ImageSharp/Memory/Allocators/UniformUnmanagedMemoryPoolMemoryAllocator.cs
@@ -1,11 +1,10 @@
-// Copyright (c) Six Labors.
+// Copyright (c) Six Labors.
 // Licensed under the Apache License, Version 2.0.
 
 using System;
 using System.Buffers;
 using System.Runtime.CompilerServices;
 using System.Runtime.InteropServices;
-using System.Threading;
 using SixLabors.ImageSharp.Memory.Internals;
 
 namespace SixLabors.ImageSharp.Memory
@@ -22,7 +21,7 @@ namespace SixLabors.ImageSharp.Memory
         private readonly int poolCapacity;
         private readonly UniformUnmanagedMemoryPool.TrimSettings trimSettings;
 
-        private UniformUnmanagedMemoryPool pool;
+        private readonly UniformUnmanagedMemoryPool pool;
         private readonly UnmanagedMemoryAllocator nonPoolAllocator;
 
         public UniformUnmanagedMemoryPoolMemoryAllocator(int? maxPoolSizeMegabytes)
@@ -74,6 +73,12 @@ namespace SixLabors.ImageSharp.Memory
             this.nonPoolAllocator = new UnmanagedMemoryAllocator(unmanagedBufferSizeInBytes);
         }
 
+#if NETCOREAPP3_1_OR_GREATER
+        // This delegate allows overriding the method returning the available system memory,
+        // so we can test our workaround for https://github.com/dotnet/runtime/issues/65466
+        internal static Func<long> GetTotalAvailableMemoryBytes { get; set; } = () => GC.GetGCMemoryInfo().TotalAvailableMemoryBytes;
+#endif
+
         /// <inheritdoc />
         protected internal override int GetBufferCapacityInBytes() => this.poolBufferSizeInBytes;
 
@@ -152,8 +157,13 @@ namespace SixLabors.ImageSharp.Memory
             // https://github.com/dotnet/runtime/issues/55126#issuecomment-876779327
             if (Environment.Is64BitProcess || !RuntimeInformation.FrameworkDescription.StartsWith(".NET 5.0"))
             {
-                GCMemoryInfo info = GC.GetGCMemoryInfo();
-                return info.TotalAvailableMemoryBytes / 8;
+                long total = GetTotalAvailableMemoryBytes();
+
+                // Workaround for https://github.com/dotnet/runtime/issues/65466
+                if (total > 0)
+                {
+                    return total / 8;
+                }
             }
 #endif
 
diff --git a/tests/ImageSharp.Tests/Memory/Allocators/UniformUnmanagedPoolMemoryAllocatorTests.cs b/tests/ImageSharp.Tests/Memory/Allocators/UniformUnmanagedPoolMemoryAllocatorTests.cs
index 45a7cc278..414f991f7 100644
--- a/tests/ImageSharp.Tests/Memory/Allocators/UniformUnmanagedPoolMemoryAllocatorTests.cs
+++ b/tests/ImageSharp.Tests/Memory/Allocators/UniformUnmanagedPoolMemoryAllocatorTests.cs
@@ -379,5 +379,20 @@ namespace SixLabors.ImageSharp.Tests.Memory.Allocators
                 g1.GetSpan()[0] = 42;
             }
         }
+
+#if NETCOREAPP3_1_OR_GREATER
+        [Fact]
+        public void Issue2001_NegativeMemoryReportedByGc()
+        {
+            RemoteExecutor.Invoke(RunTest).Dispose();
+
+            static void RunTest()
+            {
+                // Emulate GC.GetGCMemoryInfo() issue https://github.com/dotnet/runtime/issues/65466
+                UniformUnmanagedMemoryPoolMemoryAllocator.GetTotalAvailableMemoryBytes = () => -402354176;
+                _ = MemoryAllocator.Create();
+            }
+        }
+#endif
     }
 }