diff --git a/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/EntityFrameworkCore/DependencyInjection/DbContextCreationContext.cs b/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/EntityFrameworkCore/DependencyInjection/DbContextCreationContext.cs
index 2b65b199a1..779aa32391 100644
--- a/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/EntityFrameworkCore/DependencyInjection/DbContextCreationContext.cs
+++ b/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/EntityFrameworkCore/DependencyInjection/DbContextCreationContext.cs
@@ -13,7 +13,7 @@ namespace Volo.Abp.EntityFrameworkCore.DependencyInjection
 
         public string ConnectionString { get; }
 
-        public DbConnection ExistingConnection { get; set; }
+        public DbConnection ExistingConnection { get; internal set; }
 
         public DbContextCreationContext(string connectionStringName, string connectionString)
         {
diff --git a/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/Uow/EntityFrameworkCore/EfCoreTransactionApi.cs b/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/Uow/EntityFrameworkCore/EfCoreTransactionApi.cs
index ce9afacef1..5e6669a6f5 100644
--- a/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/Uow/EntityFrameworkCore/EfCoreTransactionApi.cs
+++ b/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/Uow/EntityFrameworkCore/EfCoreTransactionApi.cs
@@ -34,9 +34,10 @@ namespace Volo.Abp.Uow.EntityFrameworkCore
 
             foreach (var dbContext in AttendedDbContexts)
             {
-                if (dbContext.As<DbContext>().HasRelationalTransactionManager())
+                if (dbContext.As<DbContext>().HasRelationalTransactionManager() &&
+                    dbContext.Database.GetDbConnection() == DbContextTransaction.GetDbTransaction().Connection)
                 {
-                    continue; //Relational databases use the shared transaction
+                    continue; //Relational databases use the shared transaction if they are using the same connection
                 }
 
                 await dbContext.Database.CommitTransactionAsync(CancellationTokenProvider.Token);
@@ -54,9 +55,10 @@ namespace Volo.Abp.Uow.EntityFrameworkCore
 
             foreach (var dbContext in AttendedDbContexts)
             {
-                if (dbContext.As<DbContext>().HasRelationalTransactionManager())
+                if (dbContext.As<DbContext>().HasRelationalTransactionManager() &&
+                    dbContext.Database.GetDbConnection() == DbContextTransaction.GetDbTransaction().Connection)
                 {
-                    continue; //Relational databases use the shared transaction
+                    continue; //Relational databases use the shared transaction if they are using the same connection
                 }
 
                 await dbContext.Database.RollbackTransactionAsync(CancellationTokenProvider.FallbackToProvider(cancellationToken));
diff --git a/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/Uow/EntityFrameworkCore/UnitOfWorkDbContextProvider.cs b/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/Uow/EntityFrameworkCore/UnitOfWorkDbContextProvider.cs
index 07f1d200aa..388f1c4175 100644
--- a/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/Uow/EntityFrameworkCore/UnitOfWorkDbContextProvider.cs
+++ b/framework/src/Volo.Abp.EntityFrameworkCore/Volo/Abp/Uow/EntityFrameworkCore/UnitOfWorkDbContextProvider.cs
@@ -105,6 +105,7 @@ namespace Volo.Abp.Uow.EntityFrameworkCore
             return (TDbContext)((EfCoreDatabaseApi)databaseApi).DbContext;
         }
 
+        [Obsolete("Use CreateDbContextAsync method.")]
         private TDbContext CreateDbContext(IUnitOfWork unitOfWork, string connectionStringName, string connectionString)
         {
             var creationContext = new DbContextCreationContext(connectionStringName, connectionString);
@@ -145,6 +146,7 @@ namespace Volo.Abp.Uow.EntityFrameworkCore
             }
         }
 
+        [Obsolete("Use CreateDbContextAsync.")]
         private TDbContext CreateDbContext(IUnitOfWork unitOfWork)
         {
             return unitOfWork.Options.IsTransactional
@@ -159,6 +161,7 @@ namespace Volo.Abp.Uow.EntityFrameworkCore
                 : unitOfWork.ServiceProvider.GetRequiredService<TDbContext>();
         }
 
+        [Obsolete("Use CreateDbContextWithTransactionAsync.")]
         private TDbContext CreateDbContextWithTransaction(IUnitOfWork unitOfWork)
         {
             var transactionApiKey = $"EntityFrameworkCore_{DbContextCreationContext.Current.ConnectionString}";
@@ -191,7 +194,24 @@ namespace Volo.Abp.Uow.EntityFrameworkCore
 
                 if (dbContext.As<DbContext>().HasRelationalTransactionManager())
                 {
-                    dbContext.Database.UseTransaction(activeTransaction.DbContextTransaction.GetDbTransaction());
+                    if (dbContext.Database.GetDbConnection() == DbContextCreationContext.Current.ExistingConnection)
+                    {
+                        dbContext.Database.UseTransaction(activeTransaction.DbContextTransaction.GetDbTransaction());
+                    }
+                    else
+                    {
+                        /* User did not re-use the ExistingConnection and we are starting a new transaction.
+                         * EfCoreTransactionApi will check the connection string match and separately
+                         * commit/rollback this transaction over the DbContext instance. */
+                        if (unitOfWork.Options.IsolationLevel.HasValue)
+                        {
+                            dbContext.Database.BeginTransaction(unitOfWork.Options.IsolationLevel.Value);
+                        }
+                        else
+                        {
+                            dbContext.Database.BeginTransactionAsync();
+                        }
+                    }
                 }
                 else
                 {
@@ -239,7 +259,29 @@ namespace Volo.Abp.Uow.EntityFrameworkCore
 
                 if (dbContext.As<DbContext>().HasRelationalTransactionManager())
                 {
-                    await dbContext.Database.UseTransactionAsync(activeTransaction.DbContextTransaction.GetDbTransaction(), GetCancellationToken());
+                    if (dbContext.Database.GetDbConnection() == DbContextCreationContext.Current.ExistingConnection)
+                    {
+                        await dbContext.Database.UseTransactionAsync(activeTransaction.DbContextTransaction.GetDbTransaction(), GetCancellationToken());
+                    }
+                    else
+                    {
+                        /* User did not re-use the ExistingConnection and we are starting a new transaction.
+                         * EfCoreTransactionApi will check the connection string match and separately
+                         * commit/rollback this transaction over the DbContext instance. */
+                        if (unitOfWork.Options.IsolationLevel.HasValue)
+                        {
+                            await dbContext.Database.BeginTransactionAsync(
+                                unitOfWork.Options.IsolationLevel.Value,
+                                GetCancellationToken()
+                            );
+                        }
+                        else
+                        {
+                            await dbContext.Database.BeginTransactionAsync(
+                                GetCancellationToken()
+                            );
+                        }
+                    }
                 }
                 else
                 {